sequencing grade modified trypsin (promega)  (Promega)

Journal: iScience

Article Title: Global analysis of excitotoxicity-induced alterations in RNA structure and RNA-protein binding in neurons

doi: 10.1016/j.isci.2025.112595

Figure Lengend Snippet: PIP-Seq simultaneously detects RNA structure scores and RNA-protein binding density in primary cortical neurons revealing altered correlation of both parameters after NMDA treatment (A) Overview of PIP-Seq (Protein Interaction Profile Sequencing). Scheme of cortical neuronal primary cultures has been adapted from Servier Medical Art. (B and C) Average RBP binding (orange line) and structure scores (blue line) at each nucleotide +/− 200 nt of the annotated start and stop codon in control (B) and NMDA-treated (C) cultures for mRNAs expressed in both conditions. The tables represent Spearman’s rho correlations between RBP binding and structure scores in the indicated regions. PPSs identified in both biological replicates of control and NMDA-treated cultures were used to calculate RBP binding. Shading around the line indicates the SEM across all plotted transcripts. Gray boxes highlight the structural dips over the start and stop codon. N = 12,854 mRNAs. NS, and ∗∗∗ denote p -value >0.05, and <0.001, respectively, Spearman’s asymptotic t approximation. mRNA diagrams are representative and are not to scale.

Article Snippet: Sequencing Grade Modified Trypsin , Promega , Cat# V511A.

Techniques: Protein Binding, Sequencing, Binding Assay, Control